Application

Anti-PDCD4 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Programmed Cell Death 4 (PDCD4) is a neoplastic transformation inhibitor protein that functions as a tumor suppressor in ovarian carcinoma. The levels of PDCD4 are depleted in progressed carcinomas of the lung, breast, colon, and prostate. MA-3 domain of PDCD4 aids protein-protein interactions in eukaryotic translation initiation factor 4GI(eIF4GI) and eIF4GII with the ATP-dependent RNA helicase eIF4A. PDCD4 suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. In response to mitogens, PDCD4 is rapidly phosphorylated on Ser67 by the protein kinase ribosomal protein S6 kinase 1 (S6K1), and subsequently degraded via the ubiquitin ligase skp, cullin, f-box containing complex beta-transducin repeat-containing protein ((SCF)βTRCP). It also shows that the protein is specifically phosphorylated on Ser76 and Ser457 by p90 ribosomal S6 kinase (RSK).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Programmed Cell Death 4 (PDCD4 ) is present in the nucleus. PDCD4 gene is mapped to human chromosome 10q25.2. PDCD4 protein consists of two basic domains at the N-terminus and C-terminus and two conserved α-helical MA-3 domains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-PDCD4 specifically recognizes human, mouse, and rat PDCD4.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.